The Environmental Pathogens Information Network will be
holding a workshop entitled: Use of Indicators for Monitoring Microbial Water Quality: A
Hands on Experience on April 11, 2008 at the USGS in
The purpose of this hand-on workshop is to provide current
information on methods for recovery and tracking for professionals working in
the area of biocriteria water monitoring. This workshop will be conducted
as a hands-on exercise (lab class). By the end of
the workshop, participants should have a working knowledge of the principal
methods used in assessment of microbiology and water quality.
Topics to be covered:
• Overview of Recreational Water
Regulations
• Analytical Methods for Bacterial
Indicators
• Microbiological Sample Collection
Methods
• Pathogens and Alternate Indicators
• Quality Assurance and Quality
Control Procedures
• Rapid Methods and Microbial Source
Tracking Concepts
Registration is limited to 28 participants; we still have
a few spaces available.
For a complete workshop advertisement please access the
following link: http://www.epi-net.org/eng/Use.pdf
For registrations please access the following link: http://www.epi-net.org/eng/registration.html
Militza
Carrero-Colón
Project
Coordinator (EPI-Net)
Lilly
Hall
phone:
(765) 496-7737
Fax:
(765) 496-2926
carreroc@purdue.edu
From: owner-beachnet@great-lakes.net
[mailto:owner-beachnet@great-lakes.net] On
Behalf Of Muruleedhara Byappanahalli
Sent: Friday, March 14, 2008 9:16
AM
To: beachnet@great-lakes.net
Subject: BEACHNET==> New
articles
fyi...
Journal of
Applied Microbiology
Volume
104 Issue 4 Page 1112-1118, April 2008
H.
Kodaka, S. Mizuochi, M. Saito, H. Matsuoka (2008) Evaluation of a new medium for the enumeration of total coliforms and Escherichia coli in Japanese surface
waters
Journal of Applied Microbiology 104 (4) , 1112–1118
doi:10.1111/j.1365-2672.2007.03627.x
Aim: A
new medium, EC-Blue-10, containing chromogenic and fluorogenic substrates, KNO3
and sodium pyruvate has been developed for the rapid simultaneous detection and
enumeration of total coliforms and Escherichia
coli in water.
Methods
and Results: Two evaluations of EC-Blue-10 were carried out. Firstly,
EC-Blue-10 was compared with Colilert-MPN for 96 water samples using MPN for
total coliforms and E. coli.
Secondly, the detection of coliforms and E.
coli were compared using 2400 tubes of EC-Blue-10 and Colilert-MPN.
The regression coefficients between EC-Blue-10 and Colilert-MPN for total
coliforms and E. coli were 0·91
and 0·89, respectively. For the detection results, the Cohen’s kappa
values between the two media were 0·79 for coliforms and 0·72 for E. coli.
Conclusions: EC-Blue-10
is almost same as Colilert-MPN for the detection of coliforms and E. coli in surface waters. Further
evaluation for EC-Blue-10 is needed to verify in different geographical areas.
Significance
and Impact of the Study: EC-Blue-10 is useful method for the rapid and
simultaneous detection of total coliforms and E.
coli in water sample.
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Evaluation of
RT-PCR and reverse line blot hybridization for detection and genotyping F+ RNA
coliphages from estuarine waters and molluscan shellfish
D.C.
Love, J. Vinjé, S.M. Khalil, J. Murphy, G.L. Lovelace, M.D. Sobsey (2008)
Evaluation of RT-PCR and reverse line blot hybridization for detection and
genotyping F+ RNA coliphages from estuarine waters and molluscan shellfish
Journal of Applied Microbiology 104 (4) , 1203–1212
doi:10.1111/j.1365-2672.2007.03646.x
Aims: To
evaluate a PCR-based detection and typing method for faecal indicator viruses
(F+ RNA coliphages) in water and shellfish, and apply the method for better
understanding of the ecology and microbial source tracking potential of these
viruses.
Methods
and Results: Water and shellfish samples were collected over 3 years
at nine estuaries in the East, West and
Conclusions: RT-PCR–RLB
was a robust method for detecting and genotyping F+ RNA coliphages from diverse
coastal areas, which provided new information on the ecology of F+ RNA
coliphages.
Significance
and Impact of the Study: This performance-validated F+ RNA coliphage
method can be used for faecal indicator monitoring and microbial source
tracking, to protect recreational bathers and shellfish consumers from exposure
to pathogenic virus and their disease risks.
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Effect of
various environmental factors on the viability of Cryptosporidium parvum oocysts
R.
Reinoso, E. Becares, H.V. Smith (2008) Effect of various environmental factors
on the viability of Cryptosporidium parvum
oocysts
Journal of Applied Microbiology 104 (4) , 980–986
doi:10.1111/j.1365-2672.2007.03620.x
Aims: To
evaluate individual and combined effects of temperature (4, 18 and 25°C), pH (7
and 10), ammonia (5 and 50 mg l−1) and exposure time (1,
2, 4 and 6 days) on the viability of Cryptosporidium
parvum oocysts in water.
Methods
and Results: The viability of oocysts was evaluated using the fluorogenic
vital dyes assay (4',6-diamidino-2-phenylindole and propidium iodide). All the
factors analysed (temperature, pH, ammonia and exposure time) and their
interaction were statistically significant (P < 0·005).
Exposure of oocysts to pH 10 for 6 days at 25°C reduced oocyst viability from 
80% to 51%. Similarly, the
exposure of C. parvum oocysts to
5 mg NH3 l−1 and 50 mg NH3 l−1
for 4 days reduced their viability from between 80%
to 41·5% and 14·8%, respectively.
Conclusions: The
interaction between pH, temperature and exposure time may have adverse effects
on the survival of C. parvum
oocysts in water. Low concentrations of ammonia, as commonly found in
alga-based wastewater systems, over a long period of time can produce high C. parvum oocyst inactivation rates.
Significance
and Impact of the Study: This study provides relevant data on the
inactivation of C. parvum oocysts
in alga-based wastewater-treatment systems in the northwest of
Murulee Byappanahalli, Ph. D.
Research Microbiologist
Porter, Indiana 46304
Phone: (219) 926-8336 ext. 421
Fax: (219) 929-5792
E-mail: byappan@usgs.gov